Mainz Animal Imaging Center

Head: Jun.-Prof. Dr. Albrecht Stroh

 

 

 

 

Staff:
Andrea Kronfeld, PhD student, management MR unit
Consuelo Fois, PhD student, 2-photon microscopy staff
Pierre-Hugues Prouvot, PhD student, 2-photon microscopy staff
Michaela Moisch, MTA, laboratory management 

Platform overview:
The Mainz Animal Imaging Center (MAIC) strives to provide tools for cutting edge research in rodent models of resilience. The methods implemented at MAIC ranges from high-speed two photon imaging to high field MR imaging. In particular, MAIC focusses on the combination of optical and magnetic readout with optogenetic interrogation, allowing for implementing a causal aspect. The MRI is equipped with dedicated imaging coils, enabling the combination with optic-fiber-based readouts of neuronal population activity with optogenetic interrogation.

Current research projects:

  • Development of all optical physiology methods in 2-photon imaging
  • Implementation of virtual reality allowing for presenting behavior paradigms important for resilience while imaging cortical microcircuits
  • Establishing chronic optic fiber implantations for longitudinal of MRI applications

External collaboration partners:

  • Prof. Dr. Ralf Deichmann, Brain Imaging Center, Goethe University Frankfurt, Frankfurt/Main, Germany
  • Prof. Dr. Rainer Heintzmann, Leibniz Institute of Photonic Technology, Reg. Assoc., Jena, Germany
  • Dr. Ferruccio Pisanello, Institute of Technology Genova, Italy

Funding:

  • Boehringer Ingelheim Foundation

Key publications:

  • Schmid F, Wachsmuth L, Schwalm M, Prouvot P-H, Jubal E R, Fois C, Pramanik G, Zimmer C, Faber C, Stroh A (2016) Assessing sensory versus optogenetic network activation by combining (o)fMRI with optical Ca2+ recordings. Journal of Cerebral Blood Flow & Metabolism 36(11):1885-1900.
  • Cheng J, Sahani S, Hausrat T, Yang J-W, Ji H, Schmarowski N, Endle H, Liu X, Li Y, Böttche R, Radyushkin K, Maric H-M, Hoerder-Suabedissen A, Molnár Z, Prouvot P-H, Trimbuch T, Ninnemann O, Huai J, Fan W, Visentin B, Sabbadini R, Strømgaard K, Stroh A, Luhmann H, Kneussel M, Nitsch R, Vogt J (2016) Precise somatotopic thalamo-cortical axon guidance depends on LPA-mediated PRG-2/Radixin signaling. Neuron 92(1):126-142.
  • Vogt J, Yang JW, Mobascher A, Cheng J, Li Y, Liu X, Baumgart J, Thalman C, Kirischuk S, Unichenko P, Horta G, Radyushkin K, Stroh A, Richers S, Sahragard N, Distler U, Tenzer S, Qiao L, Lieb K, Tüscher O, Binder H, Ferreiros N, Tegeder I, Morris AJ, Gropa S, Nürnberg P, Toliat MR, Winterer G, Luhmann HJ, Huai J, Nitsch R (2016) Molecular cause and functional impact of altered synaptic lipid signaling due to a prg-1 gene SNP. EMBO Mol Med 8(1):25-38.
  • Adelsberger H, Grienberger C, Stroh A, Konnerth A (2014) In Vivo Calcium Recordings and Channelrhodopsin-2 Activation through an Optical Fiber. Cold Spring Harb Protoc 2014(10).
  • Stroh A, Adelsberger H, Groh A, Rühlmann C, Fischer S, Schierloh A, Deisseroth K, Konnerth A. (2013) Making waves: initiation and propagation of corticothalamic Ca2+ waves in vivo. Neuron 77: 1136-1150.