Mainz Animal Imaging Center

Head: Univ.-Prof. Dr. Albrecht Stroh
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Staff:

Dr. Dirk Cleppien, management MR unit
Consuelo Fois, PhD student, 2-photon microscopy staff
Pierre-Hugues Prouvot, PhD student, 2-photon microscopy staff
Michaela Moisch, MTA, laboratory management 

Platform overview:
The Mainz Animal Imaging Center (MAIC) strives to provide tools for cutting edge research in rodent models of resilience. The methods implemented at MAIC ranges from high-speed two photon imaging to high field MR imaging. In particular, MAIC focusses on the combination of optical and magnetic readout with optogenetic interrogation, allowing for implementing a causal aspect. The MRI is equipped with dedicated imaging coils, enabling the combination with optic-fiber-based readouts of neuronal population activity with optogenetic interrogation.

Current research projects:

  • Development of all optical physiology methods in 2-photon imaging
  • Implementation of virtual reality allowing for presenting behavior paradigms important for resilience while imaging cortical microcircuits
  • Establishing chronic optic fiber implantations for longitudinal of MRI applications

External collaboration partners:

  • Prof. Xin Yu, Max Planck Institute for Biological Cybernetics, Tübingen, Germany
  • Prof. Dr. Rainer Heintzmann, Leibniz Institute of Photonic Technology, Reg. Assoc., Jena, Germany
  • Dr. Ferruccio Pisanello, Institute of Technology Genova, Italy

Funding:

  • Boehringer Ingelheim Foundation

Key publications:

  • Schmid F, Wachsmuth L, Albers F, Schwalm M, Stroh A*, Faber C* (2017) True and apparent optogenetic BOLD fMRI signals. Magn Reson Med Jan;77(1):126-136. *equal contr. last authors
  • Stroh A (Editor, 2018) Optogenetics: A Roadmap. Neuromethods volume 133, SpringerNature, ISBN 978-1-4939-7415-3.
  • Schmid F, Wachsmuth L, Schwalm M, Prouvot P-H, Jubal E R, Fois C, Pramanik G, Zimmer C, Faber C, Stroh A (2016) Assessing sensory versus optogenetic network activation by combining (o)fMRI with optical Ca2+ recordings. Journal of Cerebral Blood Flow & Metabolism 36(11):1885-1900.
  • Adelsberger H, Grienberger C, Stroh A, Konnerth A (2014) In Vivo Calcium Recordings and Channelrhodopsin-2 Activation through an Optical Fiber. Cold Spring Harb Protoc 2014(10).
  • Stroh A, Adelsberger H, Groh A, Rühlmann C, Fischer S, Schierloh A, Deisseroth K, Konnerth A. (2013) Making waves: initiation and propagation of corticothalamic Ca2+ waves in vivo. Neuron 77: 1136-1150.